Verhoeff stain component: an iron-haematoxylin stain that is specific for elastic fibers. VVG is a two-part combination stain that enables differentiation of some connective tissue components in a tissue which are not easily distinguished by H&E staining: You can read more about H&E in these articles from the Microscopy and Imaging Channel- Part 1:Method and Tips, Part 2: Recipes and Materials.Īlthough there are numerous special stains for identification of elastic fibers, VVG is most commonly used because it is quick, and produces intense staining of elastic fibers. These fine elastic fibers cannot typically be seen on routine haematoxylin and eosin (H&E)-stained tissue sections, therefore special stains are required to highlight them.
#Etuner gieson skin#
They are also present in other tissues that need flexibility, such as skin and lung. What does it stain?Įlastic fibers are connective tissue fibers that allow tissues to stretch, and are abundant in the aorta, for example, where they provide flexibility to this large blood vessel. Frederick Herman Verhoeff, an American surgeon and pathologist, then modified the stain in 1908, as a method to differentiate collagen and other connective tissues, and highlight elastic fibers in particular. Ira Van Gieson first described the Verhoeff-van Gieson (VVG) staining protocol in 1889 as a method of evaluating collagen fibers in neural tissue.
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